Please clean your Submicroliter Cell and lid with a fluff-free tissue moistened with water or 70% ethanol. To avoid thawing effects make sure, that the sample has reached room temperature and it was mixed accurately before the measurement. We recommend to use purified samples to avoid disturbing signals from impurities that absorb also in the region of interest.

Make sure that your pipettes are calibrated and the required tips are used.

Please check whether you adjusted the correct program (e.g. dsDNA, RNA, oligo) for your type of sample. Please check whether the lid factor correlates with the lid and that the dilution factor has the required value (default 1.000). Review that you are working in the correct concentration range for your used lid and that the sample volume correlates to the defined amount (see average detection range sheets).

This is depending on your sample, if the concentration is below 200ng/µL in general, you start with lid 10. For higher ranges always start with lid 50 as this is covering the widest detection range. Please find the concentration ranges for each dilution lid on the application sheet: "Average detection range and required sample volume". If the concentration is not in the detection range for the used lid a warning message will appear which indicates "Abs too high" or "Abs too low". Please check the NanoPhotometer® User Manual for a detailed explanation of the messages.

Your instrument may operate with an older software version. Please perform a software update with the latest version. You can download this version from our downloads page. Your NanoPhotometer® recognizes when the concentration of the sample is too high or too low for the actual lid. You can now decide, if you like to switch to a more optimal lid or continue with the actual one. Do not hesitate to contact our support team, if assistance is required.

Within "NanoVolume Applications" and "Cuvette Applications" the values are automatically calculated. In all other methods the path length is only documented, but not calculated. You have to process the data by yourself.

Yes. Absorbance measurements are not specific for a special type of nucleic acids. The presence of other molecules that absorb also at 260nm do have influence on the results.

YES. The scratch-resistant quartz surface avoids substances to stick on the cell or onto the mirror of the lid. We recommend cleaning the cell with a slightly wet fluff-free tissue to avoid fluffs on the quartz surfaces.

In general, the Submicroliter Cell and the lids are very resistant. However, we do not recommend using concentrated acids or bases. For specific solvents, please contact the Implen support team (support@implen.de) to check the compatibility with our technology.

No. The PVC software can only be operated with Windows based PCs.

The NanoPhotometer® is designed as stand-alone unit. However, there is the possibility to transfer the data via USB, Bluetooth or SD RAM card to a computer. The software and the accessories can be purchased optional.

The latest PVC software and firmware are compatible with all versions of the NanoPhotometer®. The latest PVC software and firmware are available at http://implen.com/nanophotometer/downloads.php

The NanoPhotometer® is performing accurately over the entire lifetime as there are no moving parts that could influence the performance of the product. The patented Sample Compression Technology™ prevents the sample from evaporation. The deviation per measurements is less than ± 5% - depending on nature of sample, etc.

measurements of single sample without pipetting a fresh sample onto the system allow a maximum deviation of 0.005 Abs (fluctuation of the lamp).

The NanoPhotometer® is maintenance and service free. There is an automatic self calibration executed with every start-up.

The NanoPhotometer® works with a Xenon flash lamp. The lifetime of the lamp is approximately 10 years (10⁹ flashes). The lamp does not change intensity over its lifetime.

NO. The lamp is only active during measurements (when flashing). There will be no damage to the lamp, if you switch on the NanoPhotometer® for the whole day.

The built-in vortexer is a specially designed low vibration vortexer which has no influence on the performance of the instrument. A standard vortexer which is placed beside the NanoPhotometer® generates more vibrations to the NanoPhotometer® than the integrated low vibration vortexer.